New Research Resources Initiative by the TSCRP

The TSCRP now offers a service on our website featuring a listing of newly available TSC research resources!! Our goal is to facilitate and speed TSC research by publicizing new resources and aiding collaborations. The list displays available resources and PI contact information. Please contact the PIs directly for information and requests.

Antibodies
Award Number	PI	Products or Resources	References	Contact Information or Link
TS043009	Tomohiro Matsumoto	Polyclonal anti-rhb1 (S. pombe)	Nakase Y et al. 2006. A defect in protein farnesylation suppresses a loss of Schizosaccharomyces pombe tsc2+, a homolog of the human gene predisposed to tuberous sclerosis complex. Genetics 173(2):569-578.	tmatsumo@house.rbc.kyoto-u.ac.jp
Cell Lines
TS043006	Ariel Castro	Human LAM (lymphangioleiomyomatosis) primary cultures	Lacher MD et al. 2010. Rheb activates AMPK and reduces p27Kip1 levels in Tsc2-null cells via mTORC1-independent mechanisms: Implications for cell proliferation and tumorigenesis. Oncogene 29(50):6543-6556.	Dr. Blanca Camoretti-Mercado: bcamoret@medicine.bsd.uchicago.edu
TS043013	Kiran Chada	ELT3 cell lines with inducible TSC2 expression		chada@umdnj.edu
Computer Software
TS043017	David Sabatini	CellProfiler: Free, open-source cell image analysis software CellProfiler Analyst (formerly CellVisualizer): Software for exploring and analyzing large, high-dimensional image-derived data	Jones TR et al. 2008. CellProfiler Analyst: Data exploration and analysis software for complex image-based screens. BMC Bioinformatics 9:482. Lamprecht MR et al. 2007. CellProfiler: Free, versatile software for automated biological image analysis. Biotechniques 42(1):71-75. Carpenter AE et al. 2006. CellProfiler: Image analysis software for identifying and quantifying cell phenotypes. Genome Biology 7(10):R100.	Sabatini@wi.mit.edu http://www.cellprofiler.org/
Databases
TS043004	Steven Sparagana	Tuberous Sclerosis Complex National History Database		http://www.tsalliance.org/
Drosophila Models
TS050016	Helen McNeill	Pointed P2 transgenic Drosophila strains	McNeill H et al. 2008. Regulation of neurogenesis and epidermal growth factor receptor signaling by the insulin receptor/target of rapamycin pathway in Drosophila. Genetics 179(2):843-853.	mcneill@mshri.on.ca
TS050053	Tin Tin Su	Homozygous Tsc1 mutant drosophila larva model, an assay for drug screening		tin.su@colorado.edu
TS020015	Naoto Ito	Tsc1 and Tsc2 mutant Drosophila		iton@helix.mgh.harvard.edu
Methods
TS060052	Mark Nellist	Assay to detect pathogenic TSC1 and TSC2 variants	Coevoets R et al. 2009. A reliable cell-based assay for testing unclassified TSC2 gene variants. Eur J Hum Genet 17:301-310. Hoogeveen-Westerveld M et al. 2011. Functional assessment of variants in the TSC1 and TSC2 genes identified in individuals with Tuberous Sclerosis Complex. Human Mutation 32(4):424-435.	m.nellist@erasmusmc.nl
TS030004	Bernardo Sabatini	Hippocampal pyramidal neuronal model system to analyze loss of Tsc1	Tavazoie SF et al. 2005. Regulation of neuronal morphology and function by the tumor suppressors Tsc1 and Tsc2. Nat Neurosci 8(12):1727-1734.	bernardo_sabatini@hms.harvard.edu
Mouse Models
TS093067	Angelique Bordey	Model of tuber-like lesions: Tsc1^fl/mut	Feliciano DM et al. 2011. Single-cell Tsc1 knockout during corticogenesis generates tuber-like lesions and reduces seizure threshold in mice. J Clin Invest 121(4):1596-1607.	angelique.bordey@yale.edu
TS060030	Michael Gambello	Loss of Tsc2 in Purkinje cells: Tsc2^ko/flox, Pcp2-Cre mice Tsc2^flox/flox, Pcp2-Cre mice	Reith RM et al. 2011. Loss of the tuberous sclerosis complex protein tuberin causes Purkinje cell degeneration. Neurobiol Dis 43(1):113-122.	michael.j.gambello@uth.tmc.edu
TS043013	Kiran Chada	Tsc2 +/-, Hmga2 +/- mice Tsc2 +/-, Hmga2 -/-mice		chada@umdnj.edu
Yeast Models
TS020021	Elizabeth Henske	S. pombe strains : h-, Δtsc1::kan+ h-, Δtsc2::kan+ h+, ura4-D18, Δtsc1::kan+ h+, ura4-D18, Δtsc2::kan+ h-, leu1-32, ura-D18, ae6-216, his7-366, Δtsc1::kan+ h-, leu1-32, ura-D18, ae6-216, his7-366, Δtsc2::kan+	van Slegtenhorst M et al. 2004. Tsc1+ and tsc2+ regulate arginine uptake and metabolism in Schizo-saccharomyces pombe. J Biol Chem 279(13):12706-12713.	ep_henske@fccc.edu
TS020021	Elizabeth Henske	S. pombe strains : h+, pas1::ura4+, ura4-D18 h-, tsc1::kan+, pas1::ura4+, ura4-D18 h-, tsc2::kan+, pas1::ura4+, ura-D18 h+, leul-32, pas1::ura4+, ura4-D18 h+, leul-32, Δtsc1::kan+ h+, leul-32, Δtsc2::kan+ h+, Rhb1^G63D/S165N h+, Δtsc1::kan+, Rhb1^G63D/S165N h+, Δpas1::ura4+, Rhb1^G63D/S165N	van Slegtenhorst M et al. 2005. Pas1, a G1 cyclin, regulates amino acid uptake and rescues a delay in G1 arrest in Tsc1 and Tsc2 mutants in Schizosaccharomyces pombe. Hum Mol Genet 14(19):2851-2858.	ep_henske@fccc.edu