DEPARTMENT OF DEFENSE - CONGRESSIONALLY DIRECTED MEDICAL RESEARCH PROGRAMS

The NFRP now offers a service on our website featuring a listing of available NF research resources! Our goal is to facilitate and speed NF research by publicizing new resources and aiding collaborations. The list displays available resources and repository or PI contact information. Please contact the repository or PI directly for information and requests.

Award Number PI Product or Resource References Contact Information or Link
Cell and Molecular Bio Tools

NF100160

Toshifumi Tomoda

Plasmids for the autophagy-related protein Unc51.Atg1-myc and the NF2 point mutation Merlin K79E-myc

Flores R, Hirota Y, Armstrong B, Sawa A, Tomoda T. DISC1 regulates synaptic vesicle transport via a lithium-sensitive pathway. Neuroscience research. 2011;71(1):71-77. doi:10.1016/j.neures.2011.05.014.

ttomoda@coh.org

NF093079

Pamela Vanderzalm

Kinase domains of Hpo (aa. 1-367), MST1 (aa 1-320), and MST2(aa 1-316) in pGEX expression vector

Boggiano JC, et al. 2011. Tao-1 phosphorylates Hippo/MST kinases to regulate the Hippo-Salvador-Warts tumor suppressor pathway. Developmental Cell 21(5):888-895.

Dr. Richard Fehon
rfehon@uchicago.edu

NF093039

Jonathan Chernoff

NF1 gene repair vector designed to replace the disrupted Nf1 allele in a mouse model by restoring exon 31.

 

Jonathan.Chernoff@fccc.edu

NF080062

Lawrence Kirschner

pcDNA3.1 encoding wildtype Rac1, Rac1 Q61L, or Rac1 T17N

Manchanda P et al. 2013. Rac1 is required for Prkar1a-mediated Nf2 suppression in Schwann cell tumors. Oncogene; 32(30): 3491-3499.

Yin Z, Pringle DR, Jones GN, et al. 2011. Differential role of PKA catalytic subunits in mediating phenotypes caused by knockout of the Carney complex gene Prkar1a. Mol Endocrinol; 25: 1786-1793.

Lawrence.Kirschner@osumc.edu

NF073146

Susan Lindquist

pBabe vector encoding EGFP and pBabe vector encoding dominant-negative MEK1 mutant (Ser218Ala and Ser222Ala)

Dai C, et al. 2012. Loss of tumor suppressor NF1 activates HSF1 to promote carcinogenesis. J Clin Invest 122(10):3742-3754.

lindquist_admin@wi.mit.edu

NF043043

Wallace Ip

1. Adenoviral and lentiviral merlin and PPM1B constructs.
2. Immortalized mouse embryo fibroblasts derived from Nf2-/- mouse.
3. FRET probes of merlin conformation – C-merlin-V, C-merlin, merlin-V, C6V, C-merlinN-half, merlinC-half-V, in mammalian expression vector.
4. Deletion constructs of merlin for FRET – C-FERM-V, C-FERM-Helix-V, C-helix-CTD-V.
5. FRET probes of merlin conformation in bacterial expression vector.

Lepont et al. Point mutation in the NF2 gene of HEI-193 schwannoma cells results in expression of a non-growth suppressive merlin isoform. Mutation Research. 2008 Jan 1; 637(1-2); 142-151.

Robert.hennigan@cchmc.org

NF030070

Long-Sheng Chang

pUASP myc-tagged merlin constructs:

  • Mer+ (wild type)
  • Mer3 mutant (Met177Ile)
  • MerΔBB mutant (Blue Box deletion)
  • Mer1-379 mutant
  • Mer345-635 mutant

Dorogova NV, et al. 2008. The role of Drosophila Merlin in spermatogenesis. BMC Cell Biology Jan 10;9:1.

Yudina OS, et al. 2010. The role of the functional sites of the merlin tumor suppressor in Drosophila spermatogenesis. Russian Journal of Genetics 46(10):1214-1216.

lchang@chi.osu.edu